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KMID : 1134820190480080823
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 8 p.823 ~ p.832
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Inhibitory Effects of Platycodon grandiflorum, Codonopsis lanceolata, Corn Silk, and Acanthopanax senticosus against Nitric Oxide-Induced Cytotoxicity in SK-N-SH Cells
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Lee Hye-In
Kwak Ji-Young
Chung Mi-Ja
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Abstract
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We investigated the neuroprotective effects and action mechanism of Platycodon grandiflorum, Codonopsis lanceolata, corn silk, and Acanthopanax senticosus extracts (PG, CL, CS, and AS) against nitric oxide (NO)-induced cell death of human brain neuroblastoma SK-N-SH cells. Sodium nitroprusside (SNP) was used as the NO donor. SNP induced cell death in SK-N-SH cells, but PG, CL, CS, and AS inhibited the formation of NO and cell death in SNP-treated SK-N-SH cells. PG, CL, CS, and AS showed DPPH radical-scavenger activity in a dose-dependent manner. Heme oxygenase-1 protein levels were increased by the presence of SNP and further elevated by pretreatment with PG, CS, and AS, respectively, but not CL. SNP treatment stimulated activation of the extracellular signal-regulated kinase (Erk) and p38 pathway in SK-N-SH cells. Pretreatment with PG, CL, CS, and AS inhibited phosphorylation of Erk and p38 by SNP. Treatment with lipopolysaccharide dose-dependently increased the NO in microglia. Overactivated microglia destroyed neurons and increased NO. The effects of PG, CL, CS, and AS were shown in co-cultured cells (SK-N-SH cells and microglia). Treatment of SK-N-SH cells with PG, CL, CS, or AS exerted protective effects against SNP-induced NO production in microglia. These results suggest that PG, CL, CS, and AS could act as potential neuroprotective agents through their inhibitory effects on NO in microglia.
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KEYWORD
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heme oxygenase-1, , microglia, neuroprotective effects, nitric oxide, SK-N-SH cells
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